Oxygen is required in cellular respiration, so it is very important for aquatic life. Ap bio lab 12 dissolved oxygen college confidential. Ap biology lab manual for teachers supplement when burettes are not available, titration can be done with droppers counting drops or with syringes which can easily measure mls. Dissolved oxygen and aquatic primary productivity by. I now have a better understanding of why ecosystems create and or need dissolved oxygen and the whole process of primary productivity. The dissolved oxygen probe can be used for doing the do lab, but this is a very expensive probe. Dissolved oxygenclassic lab kit for ap biology, 3 groups. Who has answers for the questions from the ap bio college board lab 12. Biology with vernier addresses the fundamentals of a high school or college general biology course with 31 experiments that include cell respiration, photosynthesis, membrane diffusion, osmosis, human physiology, transpiration, fermentation, and more. File type pdf ap biology lab 11 answers ap biology lab 11 answers ap biology lab 11. Remind yourself of our experiment, our procedures, and our conclusions. Dissolved oxygen and primary productivity lab savage science.
Dissolved oxygen do is a measure of how much oxygen is dissolved in the water the amount of oxygen available to living aquatic organisms. The neosci dissolved oxygen and aquatic primary productivity neo lab ap biology is computerbased interactive exercises on modeling pond ecosystem productivity including dissolved oxygen do and temperature and productivity as a function of depth in a lake for advanced placement ap biology students in grades 1112, and includes a narrated tutorial, assessments of student comprehension, and customized teacher resources. Seeing that the results were consistent through multiple experiments, it is highly likely that if the experiment was repeated. In conclusion, an increase in temperature and an increase in light increase the amount of dissolved oxygen within a water sample. To explore, find and select your class from the tabs above. The difference in the dissolved oxygen levels between the uncovered tube and the covered tube is the biochemical oxygen demand of the water sample. Determine if you will use a stovetop or microwave to heat the water. September 2009 edited september 2009 in ap tests preparation. If the temperature of the water is increased, then the concentration o. Now, in your own words, explain why this calculation works. An individual license is valid for a single installation. The 1station kit includes materials for 1 group of 4 students.
While i was recently going over the dissolved oxygen data from lab 12, i realized that the nomograph in edition d page 140 of the ap lab manual is very poor and inaccurate. Effect of temperature on dissolved oxygen we actually did this part of the lab already. The amount of dissolved oxygen in a stream or lake can tell us a lot about its water quality. Ap sample lab 12 dissolved oxygen biology junction. Measure the amount of dissolved oxygen in water samples at different temperatures lab 12. From this lab i have learned that the amount of dissolved oxygen created varies on the ecosystem itself and the factors in the ecosystems that effect the dissolved oxygen level. They span the globe in their topics and their techniques. At 4,000 meters in elevation,000 feet, the amount of dissolved oxygen in water is less than twothirds what it is at sea level. Dissolved oxygen levels are used to monitor the health and productivity. Since 1989, there have been 12 laboratorieslovingly nicknamed the dirty dozenthat are suggested curricula for ap biology classes. Explain the relationship of dissolved oxygen to primary productivity. Temperature and dissolved oxygen the advanced placement biology laboratory manual recommends that each student group determine do of water samples at three different temperatures. However, more oxygen is mixed into the water with the help of winds, rain, waves, and currents.
To measure and analyze the primary productivity of natural waters of laboratory cultures using screens to simulate the decrease of light with increasing depth. In an aquatic environment, oxygen availability is influenced by a variety of chemical and physical factors. The advanced placement biology laboratory manual recommends that each student group determine do of. The effect of the temperature of water on the concentration of dissolved oxygen hypothesis. Oxygen from the atmosphere is mixed into the water through diffusion. Dissolved oxygen levels are an extremely important factor in determining the. Includes instructions on water sampling, an illustrated. These laboratory materials have been prepared by edvotek, inc. Andersen walks you through the cellular respiration lab. View lab report lab 21 dissolved oxygen and aquatic primary productive from bio 101 at bremen high school, bremen. Remove the do probe from the storage bottle and carefully insert it into the culture.
Since oxygen is one of the most easily measured products of both photosynthesis and respiration, a good way to gauge primary productivity in an aquatic ecosystem is to measure dissolved oxygen. Dissolved oxygen lesson plan template and teaching resources. We were investigating the effect of the physical factor of temperature on the percent dissolved oxygen in a body of water. Dissolved oxygen and aquatic primary productivity, answer the following questions before midnight the day before the lab or you can not do the lab. Dissolved oxygen and primary aquatic productivity laboratory 12 introduction dissolved oxygen levels are an extremely important factor in determining the quality of an aquatic environment. By engaging with these educational materials, students learn. Analysis of dissolved oxygen lab have faith in ap biology.
Some of the factors that affect the amount of oxygen dissolved in water are. Dissolved oxygen and primary productivity 6 for evaluation purposes only 39. In the dissolved oxygen classic lab kit for ap biology, the relationship between the amount of do in water and water quality are explored and quantified. The 8station kit includes enough materials for a class of 32 students working in groups of 4. We were investigating the effect of the physical factor of. Andersen demonstrates the azide winkler method of dissolved oxygen analysis. Usgs scientist is measuring various waterquality conditions in holes creek at huffman park in kettering, ohio. Would you expect the dissolved oxygen levels in water sampled from a lake at 7am to be higher or lower than the dissolved oxygen levels in water sampled at 5pm. Oxygen is used by organisms in aerobic respiration, where energy is. How would the predicted levels of do differ in each of the following pairs of water samples.
Videos anatomy and physiology ap biology ap chemistry ap environmental science ap physics biology chemistry earth. Distilled water contains no organisms, is stagnant, and is kept at room temperature. In this exercise you will be doing several calculations which analyze the dissolved oxygen concentration in water samples. Lab 21 dissolved oxygen and aquatic primary productive. The silver ring on the probe should be immersed in the culture, but the probe should not be touching the bottom of the beaker. These all contribute to a lower dissolved oxygen concentration. Dissolved oxygen and aquatic primary productivity kit.
The neosci dissolved oxygen and aquatic primary productivity neolab ap biology is computerbased interactive exercises on modeling pond ecosystem productivity including dissolved oxygen do and temperature and productivity as a function of depth in a lake for advanced placement ap biology students in grades 1112, and includes a narrated tutorial, assessments of student comprehension, and. Dissolved oxygen is necessary for the metabolic processes of almost every organism. In this exercise you will be doing several calculations which analyze the dissolved oxygen concentration in. Differences in temperature will not show differences in dissolved oxygen concentration. Terrestrial environments hold over 95% more oxygen than aquatic environments.
Dissolved oxygen prentice hall ap bio lab 12 lab bench quiz. Measure the amount of dissolved oxygen in water samples at different temperatures 2. The advanced placement biology course is the equivalent of a twosemester college introductory biology course taken by biology majors during their first year. The following citation format will be appropriate for lab reports based on one of the 12 ap biology labs. Chemicals for the winkler technique are provided in groupsized increments. Neosci dissolved oxygen and aquatic primary productivity. The faster the water moves, the more dissolved oxygen the water will contain since it has more contact time with the air. Biondo september 19, 2012 design diagram dissolved oxygen. Create a hot water bath by performing the following steps.
Definitely order the colorimeter, which can easily be used for doing the photosynthesis lab. You can save time by assigning each group one temperature of water for do determination and taking class averages. Dissolved oxygen and aquatic primary productivity lab. In the dissolved oxygen classic lab kits for ap biology, the relationship between the amount of do in water and water quality are explored and quantified using the winkler method. I compared it to a chart of 100 percent oxygen solubility at different temperatures, and in all cases for the chart values at 100 percent, the nomograph reads. It goes on for several pages so i am only paraphrasing. Andersen demonstrates the azidewinkler method of dissolved oxygen. We also predict that all of our experimental variables will contain more dissolved oxygen than our control, the distilled water.
After showing themselves to be qualified on the ap exam, some students, as college freshmen, are permitted to take upperlevel biology courses or register for courses for which biology is. Lab 21 dissolved oxygen and aquatic primary productive ap. Measurements of dissolved oxygen do are used to determine primary productivity in bodies of water. Dissolved oxygen and primary aquatic productivity laboratory 12. Andersen demonstrates the azidewinkler method of dissolved oxygen analysis. Dissolved oxygen levels are an extremely important factor in determining the quality of an aquatic environment.
Print out the completed calculation table from sample problem page of the labbench web site. We cannot measure gross productivity directly because respiration, which uses up oxygen and organic compounds, is always occurring simultaneously with. The neosci dissolved oxygen and aquatic primary productivity neolab ap biology is computerbased interactive exercises on modeling pond ecosystem productivity including dissolved oxygen do and temperature and productivity as a function of depth in a lake for advanced placement ap biology students in grades 1112, and includes a narrated tutorial, assessments of student comprehension. Always wash hands thoroughly with soap and water after handling reagents or bio. All of these physical factors, along with oxygen concentration, work together to increase diversity in aquatic habitats. The concentration of dissolved oxygen in water depends also on temperature, pressure, and concentrations of various ions cf.
Neosci dissolved oxygen and aquatic primary productivity neo. To determine if water temperature affects dissolved oxygen concentration. Videos anatomy and physiology ap biology ap chemistry. As water becomes warmer, its ability to hold oxygen. The light oxygen has 16 mg dissolved oxygen and then subtracted to 6 mg dissolved oxygen so that equals to 10 mg dissolved oxygen by net productivity. You have 5 minutes to write in your lab notebook what you understand about the element oxygen and its role in aquatic biology. The advanced placement ap program is a registered trademark of the college. Oxygen is essential for cellular respiration in most organisms.
To be successful, a method for measuring dissolved oxygen must meet two requirements. The test results indicated that after a 24hour time period, there was a difference in the primary productivity relative the amount of light tested. Dissolved oxygen and aquatic primary productivity introduction in an aquatic environment, oxygen must be dissolved in order to be available for use by organisms. The silver ring on the probe should be immersed in the culture, but the probe should not. Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the classic edition of the biology place to all educators and their students. Ap biology lab manual for teachers supplement technology integration use of gas pressure probeware provides a different means of running lab 2 a more rapidly, b without the necessity of using caustic sulfuric acid or messy permanganate stain and c without timing problems involved in stopping the reaction at specific points. After conducting this lab it was determined that primary productivity can indeed be measured using dissolved oxygen levels. I found the discussion of dissolved oxygen in one of my old limnology books to be quite helpful. Safe and compact titration assemblies providedselect from an extensive catalog of educational tools available for enhancing the teaching of biological subjects, including simulated testing of blood and urine, the illustration of various natural habitats, and the dissection of sterilized stool samples collected from different regions. The relationship between temperature, do, and respiration rates although water is composed of oxygen and hydrogen atoms, biological life in water depends upon another form of oxygenmolecular oxygen.
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